Mass-HPLC Analysis Service

  • HPLC analyses (UV-DAD, DEDL).
  • UPLC-MS and UPLC-MSMS analyses (single QUAD, Q-TOF and Q-ORBITRAP).
  • Chiral HPLC analyses – UV (normal phase and reverse phase).
  • Purification by preparative HPLC from a few milligrams to 1 gram.
  • GC-MS analyses.

EQUIPMENTS

UPLC-Q-Orbitrap EXPLORIS 120 (Thermo Scientific)

  • UPLC Vanquish – 1000 bars
  • UV and CAD detectors
  • ionization : ESI +/-; APCI +/-
  • Q-ORBITRAP, MS, MS/MS, resolution 120000
  • Available columns :

* Acquity UPLC BEH C18, Acquity Premier BEH, Acquity BEH HILIC (Waters)
* Atlantis Premier BEH C18 AX, Atlantis Premier BEH Z-HILIC (Waters)
* Zorbax Eclipse Plus C18 RRHD (Agilent Technologies)
* Kinetex Biphenyl, Kinetex PFP (Phenomenex)

UPLC-Q-TOF 6546

(Agilent Technologies)

  • UPLC 1290 Infinity – 1000 bars
  • UV detector
  • ionization : ESI +/-
  • Q-TOF, MS, MS/MS, resolution 60000
  • Available columns :
    * Acquity UPLC BEH C18, Acquity Premier BEH, Acquity BEH HILIC (Waters)
    * Atlantis Premier BEH C18 AX, Atlantis Premier BEH Z-HILIC (Waters)
    * Zorbax Eclipse Plus C18 RRHD (Agilent Technologies)
    * Kinetex Biphenyl, Kinetex PFP (Phenomenex)

UPLC-TOF 6230

(Agilent Technologies)

  •  UPLC 1260 Infinity – 800 bars
  • UV detector
  • ionization :  ESI +/-; APCI +/-
  • TOF MS, resolution 15000
  • Available columns :

* XSelect CSH C18 (Waters)
* Zorbax Eclipse Plus C18 RRHD (Agilent Technologies)
* Kinetex Biphenyl, Kinetex PFP (Phenomenex)

   LC-MSD 6125

(Agilent Technologies)

  • HPLC 1260 Infinity – 600 bars
  • UV detector
  • ionization : ESI +/-, mass range 10-2000
  • single quadrupole, MS, resolution 1000
  • Column : Cortecs T3 (Waters) – 2.7µm – 2.1x50mm.

HPLC-UV – reversed phase

  • LC 1100 series (Agilent Technologies) – binary pump 400 bars, UV detector

Available columns : 4.6x150mm – 5µm

– Xselect CSH C18 (Waters)
– Sunfire C18 (Waters)
– XBridge C18 (Waters)
– Atlantis T3 C18 (Waters)

HPLC – UV normal phase, chiral LC

  • LC Alliance 2695 (Waters) – binary pump 400 bars, UV detector

Available columns : 4.6x250mm – 5µm

– Chiralpak IA (DAICEL)
– Chiralpak IG (DAICEL)

PREP-LC ( Agilent Technologies)

  • Prep I – HPLC 1290 Infinity II- binary pump – 420 bars
    5-200mL/min – 19, 30 and 50 mm diameter columns.
  • Prep II – HPLC 1260 Infinity II – binary pump – 420 bars
    1-50mL/min – 10 and 19 mm diameter columns.
  • UV and DEDL detectors.

       

Available columns :

– Xselect CSH C18 (Waters) : 5µm – 19, 30 and 50mm diameter – length 150 mm.

– Sunfire C18 (Waters) : 5µm – 10, 19 et 30 mm diameter – length 150 mm.

– XBridge C18 (Waters) : 5µm – 19 et 30 mm diameter- length 150 mm.

– Atlantis T3 C18 (Waters) : 5µm – 19 et 30 mm diameter – length 150 mm.

GC-MSD 5977

(Agilent Technologies)

  • GC7820
  • column DB5-MS 30m
  • single quad

Team members

Scientific expert

  • Karine

    Engineer

    Responsable service Chromato-Masse

  • Somia

    Technician

  • Alexandre

    Associate Professor